Effect of trichostatin alone and combination with imatinb on the proliferation and apoptosis of the K562R cells.
10.3969/j.issn.1672-7347.2011.08.015
- Author:
Shuping CHEN
1
;
Yi LIU
;
Hui ZENG
;
Qun HE
;
Xielan ZHAO
;
Fangping CHEN
Author Information
1. Department of Hematology, Central South University, Changsha 410008, China. shuping1207@126.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Benzamides;
Cell Proliferation;
drug effects;
Drug Synergism;
Humans;
Hydroxamic Acids;
pharmacology;
Imatinib Mesylate;
K562 Cells;
Piperazines;
pharmacology;
Protein-Tyrosine Kinases;
antagonists & inhibitors;
Pyrimidines;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2011;36(8):782-785
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of trichostatin A (TSA) alone and combination with imatinib on the proliferation and apoptosis of K562R cells.
METHODS:3-(4,5-dimethylthiazol-2-yl) -5(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay was used to observe the proliferation of K562R cells and apoptosis was analyzed by annexin-V/propidium iodide(PI) staining.
RESULTS:After exposure to TSA, the proliferation of K562R cells was inhibited, and the effect was in both time- and dose-dependent manner. The apoptosis was induced. Combined with imatinib, the effect of proliferation inhibition was better.
CONCLUSION:TSA combined with imatinib can inhibit the proliferation of K562R cells and induce its apopotosis. TSA may become a new drug for imatinib-resistant patients.
