Expression of proteinase TMPRSS3 in mouse cochlea.
10.3969/j.issn.1672-7347.2011.08.018
- Author:
Shenglei GE
1
;
Qin WANG
;
Anquan PENG
;
Weijing WU
;
Dinghua XIE
Author Information
1. Department of Otolaryngology-Head & Neck Surgery, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cochlea;
metabolism;
Female;
Male;
Membrane Proteins;
genetics;
metabolism;
Mice;
Mice, Inbred C57BL;
RNA, Messenger;
genetics;
metabolism;
Serine Proteases;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2011;36(8):794-798
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the expression of proteinase transmembrane protease, serine 3 (TMPRSS3) in mouse cochlea, and to investigate the significance of TMPRSS3 in the inner ear.
METHODS:The protein expression of TMPRSS3 in C57/BL mouse cochlea was identified and detected by immunohistochemistry and immunofluorescence. Different cochlear tissues, such as spiral ganglion neurons, corti organ, stria vascularis and so on, were separated to detect the gene expression of TMPRSS3 by real-time fluorescence quantitative polymerase chain reaction (qPCR). The cochlear tissues with different ages were collected and the expression of TMPRSS3 mRNA was detected by qPCR.
RESULTS:TMPRSS3 was mainly expressed in the spiral ganglion neurons, and there was TMPRSS3 mRNA in the cochlea in groups with different age. The expression level of TMPRSS3 mRNA was much weaker.
CONCLUSION:The distribution of TMPRSS3 was observed in many regions of the mouse cochlea, but mainly in the spiral ganglion neurons. This indicates that TMPRSS3 may be involved in the physiological functional regulation of the spiral ganglion neurons.
