High fat diet induces obesity and alters the expression of MCHR1 and OB-Rb in the adipose tissue.
10.3969/j.issn.1672-7347.2011.09.002
- Author:
Jinrong LI
1
;
Jianqun YAN
;
Ke CHEN
;
Qian WANG
;
Xiaolin ZHAO
;
Yuan ZHANG
Author Information
1. Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
- Publication Type:Journal Article
- MeSH:
Adipose Tissue;
metabolism;
Animals;
Animals, Newborn;
Diet, High-Fat;
adverse effects;
Dietary Fats;
administration & dosage;
Hypothalamic Hormones;
blood;
Leptin;
blood;
Male;
Melanins;
blood;
Obesity;
etiology;
metabolism;
Pituitary Hormones;
blood;
RNA, Messenger;
genetics;
metabolism;
Rats;
Rats, Sprague-Dawley;
Receptors, Leptin;
genetics;
metabolism;
Receptors, Somatostatin;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2011;36(9):823-829
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of high-fat (HF) diet on the body weight and the mRNA expression of melanin concentrating hormone receptor 1 (MCHR1) and leptin receptor (OB-Rb) in the adipose tissue in rats, the two important and opposite factors in regulating the body weight.
METHODS:Post-weaning rats were divided into 3 groups: the NC group were fed a normal-chow diet (NC) (13% calories from fat), the HF group with a HF-diet (47% calories from fat) and the PHF group pair-fed a HF-diet (47% calories from fat). At the end of 8th week, the gained bodyweight, the plasma melanin concentrating hormone (MCH) and leptin, and the expression levels of MCHR1 and OB-Rb in the adipose tissue were measured.
RESULTS:Both the HF-diet and pair-fed HF-diet enhanced the body weight (P<0.01), plasma MCH (P<0.01) and leptin concentrations (P<0.05). In the adipose tissue, HF-diet resulted in significant increase in MCHR1 (PHF group,P<0.05) and decrease in OB-Rb mRNA levels (HF group,P<0.01; PHF group,P<0.05). No statistical difference was found between the HF group and the PHF group in terms of the aforementioned data (P>0.05).
CONCLUSION:Chronic intake of iso-caloric HF-diet and ad libitum HF-diet obviously results in increase in the body weight, serum leptin, and MCH concentration. Diet-induced obesity and related metabolic disorders are possibly correlated with up-regulated expression of MCHR1 and down-regulated expression of OB-Rb in the adipose tissue.
