Proteomics of the colonic mucosa in sub-healthy people with shapeless stool.
10.3969/j.issn.1672-7347.2011.09.001
- Author:
Baiyun ZHONG
1
;
Hui DENG
;
Jingzhong LIAO
;
Yunli ZHANG
;
Weimin XIE
;
Weidong LIU
;
Sisi FENG
Author Information
1. Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China. zbycsu@hotmail.com
- Publication Type:Journal Article
- MeSH:
Actins;
metabolism;
Aldehyde Dehydrogenase;
metabolism;
Aldehyde Dehydrogenase 1;
Annexin A5;
metabolism;
Case-Control Studies;
Colon;
metabolism;
physiopathology;
Dyspepsia;
metabolism;
Electrophoresis, Gel, Two-Dimensional;
Female;
Humans;
Hydroxymethylglutaryl-CoA Synthase;
metabolism;
Intestinal Mucosa;
metabolism;
physiopathology;
Male;
Proteins;
genetics;
isolation & purification;
metabolism;
Proteome;
analysis;
Proteomics;
methods;
Retinal Dehydrogenase
- From:
Journal of Central South University(Medical Sciences)
2011;36(9):817-822
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the 2-dimensional electrophoresis (2-DE) map in colonic mucosa in sub-healthy people with shapeless stool and healthy people, to identify the differential proteins by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), and to provide theoretical basis for the pathogenesis of intestinal mucosa in sub-healthy people with shapeless stool.
METHODS:Two-DE was used to separate the total proteins from the intestinal mucosa in sub-healthy people (the sub-health group) with the shapeless stool and healthy volunteers (the control group). ImageMaster 2D Elite soft was applied to analyze the 2-DE images, and the differentially expressed protein spots between the 2 groups were identified by MALDI-TOF-MS, protein bank and information technique.
RESULTS:We analyzed the average maps and obtained 517 protein spots in the sub-healthy group and 535 protein spots in the control group. Between the sub-healthy group and the control group, the mean of 366 protein spots was matched, and the matching rate was 70.79%. Ten differential protein spots were screened by MALDI-TOF-MS, and 8 were identified. Five out of the 8 spots were significantly decreased, while 3 out of the 8 were significantly increased.
CONCLUSION:The proteomic expression in colonic mucosa of people with shapeless stool is significantly different from that of healthy people. Eight differential proteins such as aldehyde dehydrogenase 1A1 isoform 1, 3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (mitochondrial), γ-actin, annexin A5 possibly involve in the pathogenesis of sub-healthy people with shapeless stool.
