Effects of hypoxia inducible factor-1 α siRNA on inducible nitric oxide synthase expression in HaCaT cells.
10.3969/j.issn.1672-7347.2011.10.014
- Author:
Yongjian LI
1
;
Xuyu ZU
;
Guiying ZHANG
;
Rong XIAO
;
Haiquan WEN
Author Information
1. Department of Dermatology, First Affiliated Hospital of Nanhua University, Hengyang Hunan, China.
- Publication Type:Journal Article
- MeSH:
Cell Hypoxia;
Cell Line;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
genetics;
metabolism;
Keratinocytes;
cytology;
metabolism;
Nitric Oxide Synthase Type II;
genetics;
metabolism;
RNA Interference;
RNA, Messenger;
genetics;
metabolism;
RNA, Small Interfering;
genetics
- From:
Journal of Central South University(Medical Sciences)
2011;36(10):1012-1016
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of hypoxia inducible factor -1α (HIF-1α) small interfering RNA (siRNA) on the expression of HIF-1α and inducible nitric oxide synthase (iNOS) in HaCaT cells under hypoxia.
METHODS:HaCaT cells were divided into 4 groups: the normal control group (without any treatment), the hypoxia group (under hypoxia for 24 h), the liposome control group (HaCaT cells transfected with liposome before hypoxia treatment), the RNA interference group (HaCaT cells transfected with siRNA sequences then under hypoxia for 24 h). Real-time PCR and Western blot were utilized to determine HIF-1α and iNOS mRNA and protein expression in HaCaT cells.
RESULTS:There was no significant difference of the mRNA expression of HIF-1α between the hypoxia group and the normoxia group (P>0.05), but the protein expressions of HIF-1α was increased in the hypoxic group than that in the normoxia group (P<0.05). Both the mRNA and protein expression of iNOS were increased in hypoxic conditions than that in the normoxia (P<0.05). Decreases were more significant in the mRNA and protein expression of HIF-1α and iNOS in the RNA interference group than that in the liposome control group in HaCaT cells (P<0.05).
CONCLUSION:Hypoxia increased HIF-1α and iNOS expression in HaCaT cells and inhibition of HIF-1α expression decreased iNOS expression.
