Proteomics analysis of adenosine A1 receptor agonist-induced delayed myocardial protection in rabbits.
10.3969/j.issn.1672-7347.2011.10.011
- Author:
Jianmei ZHOU
1
;
Dingquan ZOU
;
Ke RAN
;
Yetian CHANG
Author Information
1. Department of Anesthesiology, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Adenosine;
analogs & derivatives;
therapeutic use;
Adenosine A1 Receptor Agonists;
therapeutic use;
Animals;
Female;
Ischemic Postconditioning;
methods;
Male;
Myocardial Reperfusion Injury;
metabolism;
prevention & control;
Myocardium;
metabolism;
Proteome;
analysis;
Proteomics;
methods;
Rabbits
- From:
Journal of Central South University(Medical Sciences)
2011;36(10):999-1002
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes of myocardial protein expression profiles in 2-chloro-N6-cyclopentyladenosine (CCPA), an adenosine A1 receptor agonist-induced delayed myocardial protection in New Zealand rabbits .
METHODS:A total of 8 rabbits were randomly divided into a CCPA group (CCPA group) and a normal saline group (NS group). CCPA and NS were infused into rabbits in the CCPA group and the NS group respectively. Twenty-four hours later, the rabbits were subjected to 30 min left anterior descending coronary artery occlusion and were reperfused for 2 hours, then the ischemic zone tissues of left ventricle were sampled for proteomic analysis.A total of 12 other New Zeland rabbits were divided into a sham group (Sham group), a normal saline group (NS group) and a CCPA group (CCPA group). The expression of αB-crystalline, one of the differential proteins, was confirmed by Western blot.
RESULTS:Analysis of two dimensional gel electrophoresis showed that the expression of 55 protein spots were different between the two groups, 17 protein spots were preliminarily identified with the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and Mascot and Expasy bioinformatics software. These proteins included stress proteins, metabolism-associated proteins, signal transduction pathway-related proteins, ionophorous proteins, immunity-associated proteins, and so on. Western blot showed that the expression of αB-crystalline was significantly up-regulated in the CCPA group.
CONCLUSION:The myocardial protein expression profiles are changed markedly in the preconditioning late phase of CCPA .The differential proteins might be involved in the delayed cardioprotection induced by CCPA.
