Molecular genetic research of a case of Bx subgroup.
10.3969/j.issn.1672-7347.2011.12.015
- Author:
Wei LIU
1
;
Daihong LI
;
Chun LIU
Author Information
1. Department of Blood Transfusion, First Central Hospital of Tianjin, China.
- Publication Type:Case Reports
- MeSH:
ABO Blood-Group System;
genetics;
Alleles;
Base Sequence;
Blood Grouping and Crossmatching;
Exons;
genetics;
Heterozygote;
Humans;
Male;
Molecular Sequence Data;
Point Mutation;
Polymerase Chain Reaction;
methods;
Polymorphism, Single Nucleotide;
Sequence Analysis, DNA
- From:
Journal of Central South University(Medical Sciences)
2011;36(12):1213-1216
- CountryChina
- Language:Chinese
-
Abstract:
Serologic characters of Bx subtype were analyzed with standard Methods , PCR-SSP method was used for its genotype, and DNA sequences of 6 and 7 exons of ABO genes were analyzed by DNA sequencing. Serologically, the anti-B test was no or 1+, with anti-AB (1+ or 2+). The patient's auto antibodies were negative. PCR-SSP showed the patient was Bx02/O2. ABO genetic locus sequencing was 261delG in exon 6 and 297 was GG homozygous. The sample was a combination of blood group B and O2 because 297A> G, 526C> G, 657C> T, 703G> A, 796C> A, 803G> C, 905A> G, 930A> G shown by direct DNA sequencing and 803G> C, 905A> G shown by cloning DNA sequencing. The serological phenotype of the specimen was B and the genetic sequence was a combination of ABO*Bx.02.1.1 and ABO*O21.01.1.1.
