HLA-A, -B, and -DRB1 gene polymorphism in the Miao ethnic group of Hunan, China.
10.3969/j.issn.1672-7347.2010.04.001
- Author:
Xiaobing QIAN
1
;
Yubin XIE
;
Guangxiu LU
Author Information
1. Graduate School of Chinese Academy of Sciences, Beijing 100049, China.
- Publication Type:Journal Article
- MeSH:
Asian Continental Ancestry Group;
genetics;
China;
ethnology;
Gene Frequency;
Genotype;
HLA-A Antigens;
genetics;
HLA-B Antigens;
genetics;
HLA-DR Antigens;
genetics;
HLA-DRB1 Chains;
Haplotypes;
Humans;
Linkage Disequilibrium;
Polymerase Chain Reaction;
methods;
Polymorphism, Single Nucleotide
- From:
Journal of Central South University(Medical Sciences)
2010;35(4):277-285
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate HLA-A, -B, and -DRB1 gene polymorphism in the Miao ethnic group in Hunan, China.
METHODS:PCR-sequence specific oligonucleotide probes (SSO) reverse flow chip method was used to type HLA-A, -B, and -DRB1 genes of 154 unrelated healthy Miao ethnic individuals in Hunan. The allele and haplotype frequencies were calculated and compared with other populations in China.
RESULTS:A total of 10 HLA-A, 25 HLA-B, and 13 HLA-DRB1 alleles were observed in the population. The higher frequency alleles included A 11(38.96%), A 02(27.27%), A 24(18.83%), B 40(60)(17.53%), B 46(13.96%), B 15(75)(11.69%), DRB1 09(15.26%), DRB1 12(15.26%), DRB1 15(15.26%), and DRB1 04(12.66%). The most frequent haplotypes were A11-B60(9.60%), A2-B46(9.27%), A11-B75(9.22%), B75-DR12(6.31%), A11-DR12(9.62%), A11-DR4(9.07%), A11-DR15(6.69%), A11-B75-DR12(5.43%), A11-B60-DR4(4.24%), and A2-B46-DR9(3.71%). Compared with other populations in China, HLA gene polymorphism of Hunan Miao population was close to that of southern population.
CONCLUSION:HLA loci are highly polymorphic in the Miao population of Hunan, and their distribution has the character of South China population.
