Proteomic analysis of human ovarian cancer paclitaxel-resistant cell lines.
10.3969/j.issn.1672-7347.2010.04.002
- Author:
Lanqin CAO
1
;
Xin LI
;
Yi ZHANG
;
Fang PENG
;
Hong YI
;
Yan XU
;
Xinguo LI
;
Qian WANG
Author Information
1. Department of Gynecology and Obstetrics, Xiangya Hospital, Central South University, Changsha 410008, China. caolanqin@163.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
pharmacology;
Cell Line, Tumor;
Drug Resistance, Neoplasm;
genetics;
Electrophoresis, Gel, Two-Dimensional;
Female;
Humans;
NM23 Nucleoside Diphosphate Kinases;
analysis;
Ovarian Neoplasms;
genetics;
metabolism;
pathology;
Paclitaxel;
pharmacology;
Proliferating Cell Nuclear Antigen;
analysis;
Proteome;
analysis;
Proteomics;
methods;
Repressor Proteins;
analysis
- From:
Journal of Central South University(Medical Sciences)
2010;35(4):286-294
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To study the proteins related to paclitaxel-resistant of ovarian cancer cell line.
METHODS:The total proteins of paclitaxel-resistant and paclitaxel-sensitive human ovarian cancer cell lines were separated by 2-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were analyzed using image analysis software. The differential proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins.
RESULTS:Forty differentially expressed proteins were found by image analysis software, and 24 differential proteins were identified by mass spectrometry. These proteins included proliferation cell nuclear antigen (PCNA), nm23, prohibitin (PHB), annexin, alpha-enolase, heat shock protein (HSP), and so on.
CONCLUSION:Twenty-four proteins in human ovarian cancer cell lines of paclitaxel-resistant and paclitaxel-sensitive are found by proteomic techniques, which may be involved in the paclitaxel-resistance of human ovarian cancer cells.
