Neuroprotective mechanism of tetrahydroxystilbene glucoside on rats after cerebral ischemia-reperfusion.
10.3969/j.issn.1672-7347.2010.04.007
- Author:
Jie YANG
1
;
Zhiwen ZHOU
;
Qidong YANG
;
Lijun ZHENG
;
Jin ZENG
Author Information
1. Department of Neurology, Xiangya Hospital, Central South University, Changsha 410078, China. yangjie6523@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
GAP-43 Protein;
metabolism;
Glucosides;
pharmacology;
therapeutic use;
Infarction, Middle Cerebral Artery;
complications;
drug therapy;
Male;
Nerve Growth Factor;
metabolism;
Neuroprotective Agents;
pharmacology;
therapeutic use;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
prevention & control;
Stilbenes;
pharmacology;
therapeutic use
- From:
Journal of Central South University(Medical Sciences)
2010;35(4):321-328
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the neuroprotective mechanism of tetrahydroxystilbene glucoside (TSG), a Chinese medicine, on rats after cerebral ischemia-reperfusion.
METHODS:A total of 96 Sprague-Dawley male rats were divided into 4 groups (n=24): a control group, an ischemia-reperfusion (I/R) model group, a low dose TSG [60 mg/(kg.d)]group, and a high dose TSG [120 mg/(kg.d)]group. After 6 days intragastric (ig) administration of TSG or natural saline (I/R group), reversible middle cerebral artery occlusion (MCAO) model was established by intraluminal suture technique. The rats of control group were operated on while the middle cerebral artery was not blocked. At 6 h, 24 h, 48 h, and 7 d after the reperfusion, behavior test was used to evaluate the neurological deficiency of each group. The protein expressions of nerve growth factor (NGF), growth associated protein (GAP)-43, and protein kinase A catalytic subunit (PKAc) in the cortex were measured by immunohistochemical method.
RESULTS:Compared with the I/R group, the neurological defect scores of the 2 TSG groups were significantly lower except at 6 h after the reperfusion. Compared with the I/R group, the protein expression of NGF, GAP-43, and PKAc after the reperfusion of the 2 TSG groups increased significantly.
CONCLUSION:The protein expression of NGF may increase when treated with TSG after cerebral ischemia-reperfusion, which activates the PKA pathway and increases the protein expression of GAP-43 that protects the neuron.
