Phosphorylation of glycogen synthase kinase-3beta induces epithelial mesenchymal transition in human peritoneal mesothelial cells.
10.3969/j.issn.1672-7347.2010.04.008
- Author:
Min FAN
1
;
Fuyou LIU
;
Yu YANG
;
Yun YE
;
Guxiang HUANG
Author Information
1. Department of Geratology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Actins;
metabolism;
Cadherins;
metabolism;
Epithelial Cells;
cytology;
drug effects;
Epithelial-Mesenchymal Transition;
drug effects;
Glycogen Synthase Kinase 3;
metabolism;
Glycogen Synthase Kinase 3 beta;
Humans;
Lithium Chloride;
pharmacology;
Mesoderm;
cytology;
drug effects;
Peritoneum;
cytology;
Phosphorylation
- From:
Journal of Central South University(Medical Sciences)
2010;35(4):329-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of phosphorylation of glycogen synthase kinase-3beta (GSK-3beta) inducing epithelial mesenchymal transition in human peritoneal mesothelial cells (HPMC).
METHODS:Primary HPMC was harvested from human omental tissue and maintained under defined in vitro conditions. The expression of p-GSK-3beta and total GSK-3beta in HMPC was detected by Western blot after incubation with different concentrations (0, 5, 10, 20, and 40 mmol/L)of LiCl at different time points (0, 1, 3, 6, and 12 h). The protein expression of E-cadherin and alpha-SMA was also examined after treatment with 20 mmol/L LiCl according to different time courses. The intracellular distribution and expression of alpha-SMA were determined by indirect immunofluorescence.
RESULTS:LiCl stimulated phosphorylation of GSK-3beta and the effect was time-dependent and concentration-dependent to limited extent (P<0.05). The expression of alpha-SMA increased (P<0.05) and the expression of E-cadherin decreased significantly (P<0.05) after 24 h stimulation by 20 mmol/L LiCl. The indirect immunoflurescence showed that the expression of alpha-SMA in HPMC increased significantly after 24 h incubation with 20 mmol/L LiCl.
CONCLUSION:The phosphorylation of GSK-3beta leads HMPC to epithelial mesenchymal transition and provides new clue for the treatment of peritoneal fibrosis.
