Expression of VEGF-C and VEGF-D in gastric carcinoma and its relationship with lymph node metastases.
10.3969/j.issn.1672-7347.2010.04.009
- Author:
Wenhuan WANG
1
;
Renyi WU
;
Guoying SUN
;
Xinhua LI
;
Weijian YUAN
;
Li'an TANG
Author Information
1. Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Female;
Humans;
Lymphatic Metastasis;
Male;
Middle Aged;
RNA, Messenger;
genetics;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Stomach Neoplasms;
metabolism;
pathology;
Vascular Endothelial Growth Factor C;
genetics;
metabolism;
Vascular Endothelial Growth Factor D;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2010;35(4):335-340
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression of vascular endothelial growth factor (VEGF) C and D in gastric cancer and its relationship with tumor angiogenesis and lymph node metastasis.
METHODS:Immunohistochemistry(SABC) and real-time PCR were used to detect the expression of VEGF-C, VEGF-D protein and mRNA in 32 gastric cancer tissues and 32 normal gastric tissues.
RESULTS:The positive expression rate of VEGF-C and VEGF-D in gastric cancer tissue was significantly higher than that of normal gastric tissues (P<0.01), the expression of VEGF-C and VEGF-D in the gastric cancer group and the lymph node metastasis group were significantly different (P<0.05). The expression of VEGF-C and VEGF-D in gastric carcinoma was positively correlated with lymph node metastasis (P<0.01), the expression of VEGF-C and VEGF-D in well-differentiated carcinoma, moderately differentiated carcinoma and poorly differentiated carcinoma was statistically different (P<0.05). VEGF-C and VEGF-D expressions in gastric cancer cells were not related to the patient's age, sex, and lymph node distant metastasis (P>0.05).
CONCLUSION:The non-intake high expression of VEGF-C and VEGF-D in gastric cancer cells is closely related to lymph node metastasis. They serve as the important reference indicator to assess the prognosis in gastric cancer patients.
