Effect of different tidal volume ventilation on apoptosis and the expression of Bax and Bcl-2 in rat lungs.
10.3969/j.issn.1672-7347.2010.04.012
- Author:
Xi WANG
1
;
Hongwei CAI
;
Xia YAN
;
Zhi YE
Author Information
1. Department of Anesthesiology, Xiangya Hospital, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
physiology;
Female;
Lung;
pathology;
Male;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Respiration, Artificial;
adverse effects;
methods;
Tidal Volume;
physiology;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2010;35(4):351-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of different tidal volume(Vt) ventilation on apoptosis and the expression of Bax and Bcl-2 in rat lungs.
METHODS:Twenty-four healthy SD rats were randomly divided into 4 groups: a control group, a low Vt ventilation group (LV), a middle Vt ventilation group (MV), and a high Vt ventilation group (HV). Rats were subjected to different tidal volumes (10,20,and 40 mL/kg) for 2 h except the control group, which kept their own breath. We determined the lung histopathology score, W/D ratio and WBC in bronchoalceolar lavage fluid (BALF) to evaluate the lung injury and examine the apoptotic cell death, Bax and Bcl-2 protein expression by using TUNEL technique and immunohistochemistry 24 h after the operation.
RESULTS:Compared with the control group, MV and HV increased lung histopathology score, W/D ratio, WBC in BALF, apoptosis index (AI )and Bax protein expression, but decreased Bcl-2 protein expression (P<0.05). These changes showed no significant difference between the control group and the low Vt ventilation group (P>0.05).
CONCLUSION:Low Vt ventilation contributed little to apoptosis. Higher Vt ventilation can improve Bax while inhibit Bcl-2 expression to aggravate apoptosis in rat lungs.
