DJ-1 gene rearrangement mutation in patients with autosomal recessive early-onset parkinsonism using real-time PCR.
10.3969/j.issn.1672-7347.2010.05.006
- Author:
Hainan ZHANG
1
;
Bin XIAO
;
Liluo NIE
;
Jifeng GUO
;
Chunyu WANG
;
Lei WANG
;
Dan HE
;
Xinxiang YAN
;
Beisha TANG
Author Information
1. Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Age of Onset;
Child;
Child, Preschool;
China;
ethnology;
DNA Mutational Analysis;
Exons;
genetics;
Female;
Gene Rearrangement;
Humans;
Intracellular Signaling Peptides and Proteins;
genetics;
Male;
Mutation;
Oncogene Proteins;
genetics;
Parkinsonian Disorders;
genetics;
Protein Deglycase DJ-1;
Reverse Transcriptase Polymerase Chain Reaction;
Young Adult
- From:
Journal of Central South University(Medical Sciences)
2010;35(5):438-444
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a technique platform of DJ-1 gene exon rearrangement using real-time PCR and to analyze DJ-1 gene exon rearrangement mutation in patients with autosomal recessive early-onset Parkinsonism(AREP).
METHODS:Real-time PCR was used to analyze DJ-1 gene exon rearrangement mutation in 22 probands with AREP from unrelated Chinese Han families and 30 normal controls.
RESULTS:We obtained satisfactory real-time PCR reaction conditions and primers of DJ-1 gene coding exons No exon rearrangement mutation in the DJ-1 gene is detected in this group.
CONCLUSION:We established platform of DJ-1 gene exon rearrangement using real-time PCR. Exon rearrangement mutation in the DJ-1 gene is rare in Chinese patients with AREP.
