Effect of neferine combined with mdr-1shRNA on the expression of mdr-1/P-gp in K562/A02 cell line.
10.3969/j.issn.1672-7347.2010.05.007
- Author:
Qun QIN
1
;
Xibin XIAO
;
Zhaoxia XIE
Author Information
1. Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
ATP Binding Cassette Transporter, Subfamily B;
ATP Binding Cassette Transporter, Subfamily B, Member 1;
genetics;
metabolism;
Benzylisoquinolines;
pharmacology;
Drug Resistance, Multiple;
drug effects;
Drug Resistance, Neoplasm;
drug effects;
Drug Synergism;
Drugs, Chinese Herbal;
pharmacology;
Humans;
K562 Cells;
RNA, Small Interfering;
genetics;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2010;35(5):445-450
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of neferine (Nef) combined with mdr-1shRNA on the expression of mdr/P-gp in K562/A02 cell line.
METHODS:MTT assay was used to observe the cell proliferation. The expression level of P-gp was determined by Western blot and the transcription of mdr-1 gene was detected by semi-quantitative RT-PCR.
RESULTS:After K562/A02 cells were treated by Nef or mdr-1shRNA alone or both for 24 h, the proliferation of K562/A02 cells was significantly higher in the Nef combined with mdr-1shRNA treatment group than that of Nef or mdr-1shRNA alone group (P<0.01).The expression of mdr-1/P-gp in the Nef with mdr-1 shRNA group was significantly lower than that of Nef or mdr-1shRNA alone group.
CONCLUSION:Nef enhances the inhibition of mdr-1shRNA expression vector on K562/A02 cell proliferation and on P-gp protein to effectively reverse multidrug resistance induced by mdr-1 gene encoding P-gp.
