Effect of human bone morphogenetic protein 2, 3, 6, and 12 on osteosarcoma cell line UMR106.
10.3969/j.issn.1672-7347.2010.05.010
- Author:
Xingxing LI
1
;
Huanling HE
;
Limei WU
;
Wen MA
;
Yinghua CHEN
;
Jia WEI
;
Jingkun MIAO
;
Lan ZHOU
Author Information
1. Department of Biomedical Engineering, Chongqing Medical University, Key Laboratory of Biomedical Engineering of Chongqing, Chongqing 400016, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
Apoptosis;
drug effects;
Bone Morphogenetic Protein 2;
pharmacology;
Bone Morphogenetic Protein 3;
pharmacology;
Bone Morphogenetic Protein 6;
pharmacology;
Bone Morphogenetic Proteins;
pharmacology;
Bone Neoplasms;
pathology;
Cell Line, Tumor;
Cell Transformation, Neoplastic;
drug effects;
Growth Differentiation Factors;
pharmacology;
Humans;
Osteosarcoma;
pathology;
Recombinant Proteins;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2010;35(5):464-469
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of human bone morphogenetic protein (hBMPs) 2/3/6 and 12 on osteosarcoma cell UMR106.
METHODS:Adenovirus-BMP2/3/6 and 12 (AdBMP2/3/6 and12) were used to treat the cell line. Their proliferation, apoptosis, and transmigration were detected by Trypan blue exclusion test, TdT-mediated biotinylated-dUTP nick end labeling (TUNEL), acridine orange-ethidium bromide (AO/EB) double fluorescent dye staining, and transwell-room test, respectively. The alkaline phosphatase (ALP) activity was detected to reflect the differentiation of tumors.
RESULTS:Compared with the control groups, the cell survival rate of the experimental groups treated with AdBMP2/3/6 and 12 showed a significant time-dependent decrease (P<0.01). The apoptosis indexes were increased significantly (P<0.01) and the results from TUNEL and AO/EB method were consistent. The cell numbers of transmembrane significantly decreased at 24,48, and 72 h (P<0.01). AdBMP2/3/6 and 12 treatment enhanced the activity of ALP activity from day 3 and this effect might still be observed up to day 9 of the treatment (P<0.01).
CONCLUSION:hBMPs2/3/6 and 12 can inhibit the proliferation and transmigration, and induce their apoptosis and differentiation in osteosarcoma cell line UMR106.
