Change of p-CREB after surgical incisional pain in rat spinal cord.
10.3969/j.issn.1672-7347.2010.08.010
- Author:
Yulong CUI
1
;
Junmei XU
;
Ruping DAI
;
Changqi LI
;
Dan LIU
;
Longqiu YANG
;
Juan WANG
Author Information
1. Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cyclic AMP Response Element-Binding Protein;
metabolism;
Female;
Foot;
surgery;
Neurons;
metabolism;
Pain Threshold;
Pain, Postoperative;
metabolism;
Phosphorylation;
Rats;
Rats, Sprague-Dawley;
Spinal Cord;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2010;35(8):833-839
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the role of phosphorylation of cAMP response element binding protein (CREB) in the incision-induced pain hypersensitivity.
METHODS:A longitudinal incision was made in one plantar hind paw of isoflurane-anesthetized rats. Spinal cords were removed at various postoperative time after behavior test. Phosphorylation of CREB was determined by immunohistochemistry and double-labeling immunofluorescence. Morphine and gabapentin were intraperitoneally injected before the behavior test and were used to determine the interaction between phosphorylation of CREB and morphine and gabapentin.
RESULTS:After the hind-paw incision, phosphorylation of CREB was enhanced in the ipsilateral lumbar spinal cord (P<0.05). The enhancement of p-CREB was mainly in the neurons in the dorsal horn of the spinal cord. All these were shown by double-labeling technique and p-CREB was mainly in the neurons. Intraperitoneal injection of morphine prevented the increased phosphorylation of CREB in the spinal cord and inhibited the mechanical allodynia induced by the incision (P>0.05). Gabapentin didn't inhibit the phosphorylation of CREB (P<0.05) but partly inhibited the mechanical allodynia.
CONCLUSION:Incision induces the phosphorylation of CREB in the spinal cord, and the increase of p-CREB is mainly in the neurons. Phosphorylation of CREB in the spinal cord contributes to the pain hypersensitivity induced by surgical incision.
