Effect of GM6001 on the model that cervical squamous carcinoma cell line HCE1 multicellular spheroids invade live human umbilical vein endothelium cell monolayers.
10.3969/j.issn.1672-7347.2010.08.016
- Author:
Jing LAN
1
;
Yilin WU
Author Information
1. Department of Gynecology and Obstetrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma, Squamous Cell;
pathology;
Cell Line;
Cell Line, Tumor;
Dipeptides;
pharmacology;
Endothelial Cells;
pathology;
Female;
Humans;
Matrix Metalloproteinase 9;
metabolism;
Neoplasm Invasiveness;
Spheroids, Cellular;
metabolism;
pathology;
Umbilical Veins;
cytology;
Uterine Cervical Neoplasms;
pathology
- From:
Journal of Central South University(Medical Sciences)
2010;35(8):868-874
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between matrix matrix metalloproteinases (MMP)-9 expression in HCE1/multicellular spheroids (MCS) and invasion of cervical carcinoma, and to explore the effect of MMPs inhibitor GM6001 on the model that HCE1/MCS invade live human umbilical vein endothelium cell (HUVEC).
METHODS:We established the invasion model by coculturing HCE1/MCS and HUVEC, and detected the MMP-9 expression in HCE1 monolayer cells (HCE1/MC), HCE1/MCS and HUVEC using immunohistochemistry and treated the HCE1/MCS invasion model with GM6001.
RESULTS:A cervical squamous carcinoma cell HCE1 infiltrating model was established. Compared with that in HCE1/MC, MMP-9 expression in the HCE1/MCS was significantly higher (P<0.05). Compared with the control group, the invasion of HCE1/MCS was inhibited by 26.09% and 92.95% (P<0.05) in the GM6001 2.5 μmol/L and 12.5 μmol/L group. MMP-9 expression in HCE1/MCS in the GM6001 12.5 μmol/L group was obviously lower than that in the control group (P<0.05). With the increase of the concentration of GM6001, the inhibition increased.
CONCLUSION:The enhancement of HCE1/MCS invasion may be related to the up-regulation of MMP-9 expression in HCE1/MCS. GM6001 can down regulate the MMP-9 expression in HCE1/MCS and partially block the HCE1/MCS invasion to HUVEC.
