Expression of checkpoint kinase 1 and polo-like kinase 1 and its clinicopathological significance in benign and malignant lesions of the stomach.
10.3969/j.issn.1672-7347.2010.10.008
- Author:
Hongliang YAO
1
;
Zhulin YANG
;
Yongguo LI
Author Information
1. Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Adenocarcinoma;
metabolism;
Adult;
Aged;
Biomarkers, Tumor;
metabolism;
Cell Cycle Proteins;
metabolism;
Checkpoint Kinase 1;
Female;
Gastritis;
metabolism;
Humans;
Lymphatic Metastasis;
Male;
Middle Aged;
Protein Kinases;
metabolism;
Protein-Serine-Threonine Kinases;
metabolism;
Proto-Oncogene Proteins;
metabolism;
Stomach Neoplasms;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2010;35(10):1080-1084
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the expressive level of checkpoint kinase 1 (CHK1) and polo-like kinase 1 (PLK1) and to detect their clinicopathological significance in benign and malignant lesions of the stomach.
METHODS:Envision Tm immunohistochemistry was used to detect the expression level of CHK1 and PLK1 in conventional paraffin-embedded sections from specimens of primary foci (n=59)and metastatic foci of lymph node (n=42) of gastric cancer, peritumoral tissues (n=20), and benign lesions of the stomach (n=95).
RESULTS:The positive rates of CHK1 were significantly higher in gastric cancer than that in different types of benign lesions(P<0.01). The positive rates of PLK1 were significantly higher in gastric cancer than that in peritumoral tissues (P<0.05) and different types of benign lesions (P<0.01), and the positive cases of PLK1 in benign lesion showed atypical hyperplasia. No significant difference of CHK1 and PLK1 expression was found between metastatic foci and corresponding primary foci (P>0.05). The positive rates of CHK1 and PLK1 were significantly lower in the non-metastatic lymph node than that in the metastatic lymph node (P<0.05). The positive rate of CHK1 was significantly lower in histologic grade II than that in the histologic grade III+IV (P<0.05). Positive correlation was found between the expression of CHK1 and PLK1 in gastric cancer tissues (P<0.01).
CONCLUSION:The expression level of CHK1 and /or PLK1 might be important biological markers of kinases to reflect the carcinogenesis, progression, biological behaviors, and guide clinical auxiliary treatment of gastric cancer.
