Effect of celecoxib and adriamycin on proliferation and apoptosis of Raji cells and its mechanism.
10.3969/j.issn.1672-7347.2010.10.009
- Author:
Xiaochun HUANG
1
;
Xiaolin LI
;
Ranxin HUANG
;
Xiaogang YANG
;
Bin FU
Author Information
1. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Burkitt Lymphoma;
pathology;
Caspase 9;
genetics;
metabolism;
Celecoxib;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Dose-Response Relationship, Drug;
Doxorubicin;
pharmacology;
Drug Synergism;
Humans;
Pyrazoles;
pharmacology;
RNA, Messenger;
genetics;
metabolism;
Sulfonamides;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2010;35(10):1085-1090
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To assess the effect of celecoxib in combination with adriamycin(ADM) on cell proliferation and apoptosis.
METHODS:Cell lines were treated with celecoxib,and cell proliferation was examined by the method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) at different time. The cellular apoptotic rate was analyzed by flow cytometry. RT-PCR was applied to distinguish the level of caspase-9 mRNA expression. Immunohistochemistry was used to detect the caspase-9 protein expression in Raji cells.
RESULTS:Celecoxib inhibited the proliferation and increased the apoptosis of Raji cell lines in a dose-dependent and time-dependent manner within 20-100 μmol/L. Compared with the celecoxib alone, the proliferation of Raji cell lines incubated with celecoxib and adriamycin was decreased. Caspase-9 mRNA and protein expression in Raji cells were significantly enhanced after the treatment of celecoxib (P<0.05).
CONCLUSION:Celecoxib can inhibit the proliferation of Raji cells in a dose-dependent and time-dependent manner. Celecoxib may lead to the apoptosis of Raji cells by up-regulating activities of caspase-9. Adriamycin could intensify the effect.
