SARA expression in high glucose induced epithelium to mesenchymal transition of proximal tubule cells.
10.3969/j.issn.1672-7347.2010.12.005
- Author:
Wenbin TANG
1
;
Guanghui LING
;
Lin SUN
;
Youming PENG
;
Yinghong LIU
;
Hong LIU
;
Li XIAO
;
Shaobin DUAN
;
Fuyou LIU
Author Information
1. Department of Nephrology, Second Xiangya Hospital, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cells, Cultured;
Epithelial Cells;
cytology;
Glucose;
pharmacology;
Humans;
Intracellular Signaling Peptides and Proteins;
genetics;
metabolism;
Kidney Tubules, Proximal;
cytology;
metabolism;
Membrane Proteins;
metabolism;
Mesoderm;
cytology;
Phosphoproteins;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Serine Endopeptidases;
genetics;
metabolism;
Signal Transduction;
Transforming Growth Factor beta1;
pharmacology;
Vimentin;
metabolism;
Zonula Occludens-1 Protein
- From:
Journal of Central South University(Medical Sciences)
2010;35(12):1230-1235
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct the cell model of epithelium to mesenchymal transition of proximal tubule cells induced by high glucose and to determine the expression of Smad anchor for receptor activation (SARA).
METHODS:Protein expression of vimentin, Zona occludens-1(ZO-1), and SARA was determined by Western blot, and their mRNA expressions were detected by Real-time PCR.
RESULTS:After stimulation by 30 mmol/L D-glucose, the protein and mRNA expression levels of vimentin in HK-2 cells increased in a time-dependent manner while the expression of ZO-1 was reduced significantly, especially at 48 h. Meanwhile, SARA was also decreased in a time-dependent manner.
CONCLUSION:High glucose can induce renal epithelium to mesenchymal transition, and SARA may be involved in this process as a protector.
