Effect of nylestriol and levonorgestrel on the expression of estrogen receptor subtypes in human osteosarcoma MG-63 cell lines.
10.3969/j.issn.1672-7347.2010.12.008
- Author:
Kaichu YANG
1
;
Eryuan LIAO
;
Houde ZHOU
Author Information
1. Department of Internal Medicine, Shenzhen Hospital of Armed Police, Shenzhen Guangdong 518029, China. ykc0076@sina.com
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Estrogen Receptor alpha;
genetics;
metabolism;
Estrogen Receptor beta;
genetics;
metabolism;
Humans;
Levonorgestrel;
pharmacology;
Osteosarcoma;
metabolism;
pathology;
Quinestrol;
analogs & derivatives;
pharmacology;
RNA, Messenger;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2010;35(12):1248-1253
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of different concentrations of nylestriol (NYL) and levonorgestrel (LNG) on the expression of ERα and ERβ in human osteoscarcoma MG-63 cell lines, and to explore the impact of paracrine effect on the gene expression.
METHODS:MG-63 cells were treated with 3 concentrations (10(-10),10(-8), and 10(-6) mol/L) of NYL or LNG. The untreated control group and the positive control group were also established. The 2 groups treated with NYL (10(-10) mol/L) or LNG (10(-8) mol/L) were designed to renew the medium every 12 h. Semi-quantitative RT-PCR was conducted to detect the mRNA expression of ERα and ERβ on the MG-63 cells treated with different concentrations of the 2 drugs, respectively.
RESULTS:Both drugs up-regulated ERα and ERβ mRNA expression. The best concentration for both NYL and LNG was 10(-6) mol/L for ERα expression. As for ERβ, the best concentration of NYL and LNG was 10(-10) mol/L and 10(-8) mol/L. The role of medium replacement on the expression of ERα was not observed, but medium replacement inhibited ERβ expression.
CONCLUSION:Both NYL and LNG can up-regulate the mRNA expression of ER subtypes in MG-63 cells, with mutual restriction between the 2 subtypes. The paracrine effect on MG-63 cell lines may be involved in the regulation process of mRNA expression of ERβ.
