Effect of pregnancy-associated plasma protein-A on the function of endothelial cells.
10.3969/j.issn.1672-7347.2010.12.010
- Author:
Liping PENG
1
;
Jiangang WANG
;
Kan YANG
;
Weihong JIANG
;
Wenguang YAN
;
Bin YI
Author Information
1. Department of Cardiovasology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Endothelial Cells;
cytology;
metabolism;
physiology;
Endothelin-1;
biosynthesis;
Female;
Humans;
Nitric Oxide;
biosynthesis;
Pregnancy-Associated Plasma Protein-A;
pharmacology;
Umbilical Veins;
cytology;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2010;35(12):1261-1265
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of pregnancy-associated plasma protein A (PAPP-A) on the function of vascular endothelial cells (VEC).
METHODS:Human umbilical vein endothelial cell (HUVEC) line, derived from human umbilical vein, was cultured in vitro with PAPP-A at 0, 50, 100, and 200 ng/mL for 0, 12, 24, and 48 hours, respectively. Nitric oxide (NO) levels and endothlin-1 (ET-1) levels were determined by spectrophotometer and immunehistory.
RESULTS:The NO levels of HUVECs in the PAPP-A groups were significantly lower than those in the control group (P<0.05). The ET-1 levels of HUVECs in the PAPP-A groups were significantly higher than those in the control group (P<0.05). The changes were all dose-dependent.
CONCLUSION:PAPP-A may affect the function of vascular endothelial cells by reducing the secretion of NO and increasing the level of ET-1.
