Interaction of olfactory ensheathing cells with nerve repairing scaffolds.
- Author:
Yonghong WANG
1
;
Yonghong WANG
;
Yixia YIN
;
Shipu LI
;
Qiongjiao YAN
;
Zhitao WAN
;
Yingchao HAN
Author Information
1. Department of Internal Medicine, Hospital of Wuhan University of Technology, Wuhan 430070, China. wangyonghong02@sina.com
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Biocompatible Materials;
Cells, Cultured;
Lactic Acid;
chemical synthesis;
pharmacology;
Nerve Regeneration;
Olfactory Bulb;
cytology;
Polyglycolic Acid;
chemical synthesis;
pharmacology;
Polylactic Acid-Polyglycolic Acid Copolymer;
Rats;
Rats, Wistar;
Spinal Cord Injuries;
physiopathology;
Tissue Engineering;
methods;
Tissue Scaffolds;
chemistry
- From:
Journal of Central South University(Medical Sciences)
2009;34(5):382-387
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate a new way to yield plenty of high purity olfactory ensheathing cells (OECs) and its biocompatibility with appropriate scaffolds.
METHODS:OECs were prepared from neonatal Wister rats and co-cultured with poly [LA-co-(Glc-alt-Lys)] (PLGL). Its contact angle, adherent rate, and activity rate were tested.
RESULTS:The contact angle of poly (D, L-lactic acid) (PDLLA) (84.5 degree+/-1.5 degree) was significantly higher than that of PLGL (52.6 degree+/-0.8 degree), the adherent rate of PLGL (80%) was significantly higher than that of the PDLLA (57%), and the activity rate of PLGL (88%) was much higher than that of the PDLLA (76%).
CONCLUSION:PLGL possesses better hydrophilicity and biocompatibility than PDLLA, and it can provide a better cell growth circumstance which is helpful for the effective treatment of nerve injury.
