Establishment of protein expression profile of laser capture microdissection-purified nasopharyngeal carcinoma tissue.
- Author:
Fang PENG
1
;
Can'e TANG
;
Maoyu LI
;
Cui LI
;
Ailan CHEN
;
Feng LI
;
Pengfei ZHANG
;
Meixiang LI
;
Zhiqiang XIAO
;
Zhuchu CHEN
Author Information
1. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Computational Biology;
Gene Expression Profiling;
Humans;
Lasers;
Microdissection;
methods;
Nasopharyngeal Neoplasms;
genetics;
metabolism;
Neoplasm Proteins;
genetics;
isolation & purification;
metabolism;
Proteome;
metabolism;
Proteomics;
methods;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From:
Journal of Central South University(Medical Sciences)
2009;34(6):481-486
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the protein expression map of nasopharyngeal carcinoma (NPC), and provide a basis for proteomic study of NPC.
METHODS:Laser capture microdissection (LCM) was used to isolate cancer cells from NPC tissues. Two-dimensional gel electrophoresis(2-DE) was used to separate the total proteins of LCM purified NPC cells. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) was performed to identify the proteins, and bioinformatics was used to construct the 2-DE database of NPC proteome.
RESULTS:A 2-DE reference map of NPC was established. On the 2-DE map, a total of (1 312+/-30) protein spots were detected, and 427 protein spots representing 241 non-redundant proteins were identified. The 2-DE database of NPC proteome was constructed. These data could be accessed at our website (http://www.xyproteomics.org).
CONCLUSION:A protein expression profile of LCM purified NPC tissues has been established for the first time, which provides useful information and source for proteomic study of NPC.
