Identification of active efflux system gene qacA/B in methicillin-resistant Staphylococcus aureus and its significance.
- Author:
Rong ZHENG
1
;
Min WANG
;
Bin HE
;
Xianping LI
;
Hong CAO
;
Hao LIANG
;
Zhiju QING
;
Aiguo TANG
Author Information
1. Department of Clinical Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Bacterial Proteins;
analysis;
genetics;
Base Sequence;
Drug Resistance, Multiple;
genetics;
Membrane Transport Proteins;
analysis;
genetics;
Methicillin-Resistant Staphylococcus aureus;
genetics;
Molecular Sequence Data;
Polymerase Chain Reaction;
methods
- From:
Journal of Central South University(Medical Sciences)
2009;34(6):537-542
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the active efflux gene qac gene in methicillin-resistant Staphylococcus aureus (MRSA) by hem-nested polymerase chain reaction (PCR) and to learn the carrier condition of qac gene.
METHODS:The active efflux gene qacA/B and qacB of 80 strains MRSA isolated from clinical specimens from Aug 2006 to March 2008 were amplified in vitro by hem-nested PCR with the primers designed by computers based on qac information of Genbank, and the PCR fragments were sequenced and analyzed.
RESULTS:We detected qacA/B in 19 out of the 80 MRSA strains (23.75%) and qacB in 18 out of the 80 MRSA strains (22.5%). Compared with sequences of qacA (NO.X56628) and qacB(NO.AF535087) in the Genbank, 98% and 97% were identical, respectively.
CONCLUSION:The active efflux gene qac gene in MRSA is detected by hem-nested PCR. The proportion of qac gene positive strains is high in clinical practice, which is related to its multi-antibiotic resistance.
