Effect of gamma-aminobutyric acid on malignant phenotypes of hepatocellular carcinoma cell line HepG-2.
- Author:
Yuehui LI
1
;
Yan LIU
;
Jiajia WANG
;
Jinyue HU
;
Guohua ZHOU
;
Pingli XIE
;
Guancheng LI
Author Information
1. Cancer Research Institute, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Cycle;
drug effects;
Cell Proliferation;
drug effects;
Dose-Response Relationship, Drug;
Hep G2 Cells;
Humans;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Neoplasm Transplantation;
Phenotype;
gamma-Aminobutyric Acid;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2009;34(8):752-756
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of gamma-aminobutyric acid(GABA) on proliferation and malignant phenotypes of hepatocellular carcinoma cell line HepG-2.
METHODS:HepG-2 cells were cultured by routine method, and then treated with different concentrations of GABA. The proliferation of HepG-2 cells was measured through MTT, doubling time and cell cycles by flow cytometry. The malignant phenotypes were investigated by soft agar colony formation assay.
RESULTS:Compared with the control group, GABA efficiently stimulated the proliferation of HepG-2 cells in a dose-dependent manner and affected the distribution of cell cycles of HepG-2 cells. The doubling time of the control group and the GABA-treated group were 39.0, 30.6, 30.0, 27.3, 26.6, and 38.2 h, respectively. The colony formation rates were 3.2%, 4.2%, 5.4%, 6.6%,6.5%, and 3.5%, respectively. Tumorigenicity testing showed that the average weights of tumor was 1.382 g, and 0.285 g for the 2 groups. The difference between the control group and the GABA-treated groups was significant (P<0.01).
CONCLUSION:GABA can enhance the proliferation and malignant phenotypes of HepG-2 cells.
