Differential proteomic analysis of thalamus in rats with diffuse axonal injury.
- Author:
Zhenyan LI
1
;
Yunsheng LIU
;
Su'e WANG
;
Junyu WANG
;
Guangwei ZHONG
;
Wei LI
;
Zhixiong LIU
Author Information
1. Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Diffuse Axonal Injury;
metabolism;
Electrophoresis, Gel, Two-Dimensional;
Male;
Proteome;
analysis;
Proteomics;
methods;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Thalamus;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2009;34(8):785-789
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To examine the differential expression of protein of thalamus in rats with diffuse axonal injury.
METHODS:Twenty-five rats were randomly divided into a normal group (n=10) and a trauma group (n=15). Total proteins of brain trauma tissue and normal brain tissue were extracted separately, and then proteins were separated by two dimensional gel electrophoresis and stained with Coomassie brilliant blue. The differentially expressed protein spots were identified with biospectrometry. Images were analyzed by PDQuest 7.0.
RESULTS:The distribution of protein spots in the trauma group was similar to that of the normal group, the matching rate was 95%, and the repeatability was good. Proteins were mainly displayed at pI 3-8, with relative molecular mass 14.4-75.0 kD. Compared with the normal group, 16 spots of proteins increased and 18 spots of proteins decreased in the trauma group.
CONCLUSION:There is some difference in protein expression between the normal group and the trauma group. Brain trauma may lead to changes of proteins in the thalamus.
