Expression of plasma thrombospondin-1 in renal interstitial fibrosis.
- Author:
Yuncheng XIA
1
;
Jing NIE
;
Zhilan LI
;
Lin SUN
;
Yinhong LIU
;
Guanghui LING
;
Youmin PENG
;
Fuyou LIU
Author Information
1. Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Fibrosis;
metabolism;
pathology;
Glomerulosclerosis, Focal Segmental;
pathology;
Kidney;
metabolism;
pathology;
Male;
Nephrectomy;
Nephritis, Interstitial;
etiology;
metabolism;
pathology;
Rats;
Rats, Sprague-Dawley;
Thrombospondin 1;
blood
- From:
Journal of Central South University(Medical Sciences)
2009;34(8):796-802
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the expression of plasma thrombospondin-1(TSP-1) at different time in protein-overload rats and to analyze the relationship between plasma TSP-1 expression and renal interstitial fibrosis.
METHODS:Forty-five male Sprague-Dawley rats were randomly divided into a bovine serum albumin (BSA) group and a control group after uninephrectomization. Rats with protein overload nephropathy induced by intraperitoneally injected BSA were used as a model (control group received saline). At the 1st, 5th, and 9th weekend, the level of 24 h proteinuria and renal function was assessed. Pathological changes were observed by electron and fluorescent microscopy. The expression of plasma TSP-1 was detected by Western blot. The relationship between plasma TSP-1 and tubulointerstitial lesions (TIL) score was analyzed.
RESULTS:Twenty-four hour proteinuria and blood urea nitrogen (BUN) significantly increased in protein-overload rats compared with those in the control group. While protein-overload rats developed more severe fibrosis in the tubular and interstitium. Glomerulosclerosis index and TIL score were upregulated compared with those in the control group. The expression of TSP-1 increased significantly at the 5th and 9th weekend. The expression of TSP-1 was positively correlated with TIL score (r=0.836, P<0.01).
CONCLUSION:Plasma TSP-1 expression is positively correlated with renal interstitial fibrosis in protein-overload rats. Plasma TSP-1 may be used for an important biomarker of renal interstitial fibrosis.
