Identification of EBV chromosomal integration sites in Raji cells by fluorescence in situ hybridization.
- Author:
Jianming GAO
1
;
Xiaoling LI
;
Guiyuan LI
Author Information
1. Department of Pathophysiology, School of Medicine, Three Gorges University, Yichang Hubei 443002,China.
- Publication Type:Journal Article
- MeSH:
Burkitt Lymphoma;
genetics;
pathology;
virology;
Cell Line, Tumor;
Chromosomes, Human, Pair 1;
virology;
Chromosomes, Human, Pair 2;
virology;
Chromosomes, Human, Pair 4;
virology;
DNA, Viral;
genetics;
Genome, Viral;
Herpesvirus 4, Human;
genetics;
Humans;
In Situ Hybridization, Fluorescence;
Virus Integration;
genetics
- From:
Journal of Central South University(Medical Sciences)
2009;34(1):13-19
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify the Epstein-Barr virus (EBV) chromosomal integration sites in Raji cells.
METHODS:EBV DNA was detected by Southern hybridization, and the viral chromosomal integration sites were identified using G banding and fluorescence in situ hybridization (FISH).
RESULTS:BamHI-digested genomic DNA from Raji cells was hybridized with (32)P-labeled probe-1 (EBV genome 13,232 approximately 16,189) and Probe-2 (EBV genome 5 approximately 3,271), which generated 4 and 10, 23 kb positive bands respectively. The viral integration sites included 1p, 1q, 2q, 3p, 3q, 4 q, 5q, 6q, 7p, 7q, 9q,11p, 14 q, and 15q,and chromosomal bands 4 q, 2q, 1q and 7q were viral integration sites with high frequencies. Among the 33 signals counted, 7, 4, 4,and 4 signals were at the site 4 q, 2q, 1q, and 7q respectively, and 64% of the total signals were found in these 4 chromosomal bands. No viral integration occurred in chromosomes 16 approximately 22 or the sex chromosomes (X, Y).
CONCLUSION:This study firstly identifies the EBV integration sites in Raji cells using G banding and FISH. There are some viral integration sites with high frequencies in Raji cells, and EBV integrates into Raji cell genomes non-randomly.
