Resistance by hypermutable Pseudomonas aeruginosa and beta-lactamases production.
- Author:
Wei CAO
1
;
Dongmei YAO
;
Rong ZHENG
Author Information
1. Department of Clinical Laboratory,Second Xiangya Hospital,Central South University, Changsha 410011, China. cwcao10741@sohu.com
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Bronchitis, Chronic;
microbiology;
Drug Resistance, Multiple, Bacterial;
genetics;
Female;
Humans;
Male;
Middle Aged;
Mutation;
Pseudomonas aeruginosa;
drug effects;
enzymology;
genetics;
Pulmonary Heart Disease;
microbiology;
beta-Lactamases;
biosynthesis
- From:
Journal of Central South University(Medical Sciences)
2009;34(1):54-58
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the relation between resistance of hypermutable Pseudomonas aeruginosa and beta-lactamases produced.
METHODS:The bacteria cultured were identified with API 20NE system. Susceptibilities of the bacteria were detected by disk diffusion method. The hypermutable strains were tested with broth dilution assays. The beta-lactamases produced by these strains were characterized by 3-dimensional test and 2-mercaptopropanoic acid inhibited assays.
RESULTS:Altogether 120 strains were analyzed and 45 (37.5%) trains were hypermutable.The resistant rates of hypermutable strains were close to or above 60.0% for imipenem, meropenem, cefoperazone/sulbactam, piperacillin/ tazobactam, ceftazidime, cephfime, aztreonam, amikacin and ciprofloxacin.The 3-dimensional test showed that 18 (40.0%) strains produced extended spectrum beta-lactamases (ESBLs), 25 (55.6%) strains produced AmpC enzymes, and 6 (13.3%) strains produced metallo-beta-lactamases.
CONCLUSION:The resistant rates of hypermutablce strains of Pseudomonas aeruginosa to routine antibiotics are high, which is one of the most important reasons for multi-drug resistance that the hypermutable strains produced ESBLs, AmpC enzymes, and metallo-beta-lactamases.
