Effect of atorvastatin on lipopolysaccharide-induced expression of C-reactive protein in human pulmonary epithelial cells.
- Author:
Shangjie WU
1
;
Xiqian XING
;
Ye GAN
;
Shuiping ZHAO
;
Ping CHEN
Author Information
1. Department of Respiratory Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China. wu_shangjie@hotmail.com
- Publication Type:Journal Article
- MeSH:
Atorvastatin;
C-Reactive Protein;
genetics;
metabolism;
Cell Line;
Down-Regulation;
drug effects;
Epithelial Cells;
metabolism;
pathology;
Heptanoic Acids;
pharmacology;
Humans;
Lipopolysaccharides;
antagonists & inhibitors;
Lung;
metabolism;
pathology;
Pyrroles;
pharmacology;
RNA, Messenger;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2009;34(2):104-108
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of atorvastatin on lipopolysaccharide-induced expression of C-reactive protein in cultured A549 cells.
METHODS:A549 cells were incubated in DMEM medium containing lipopolysaccharide in the absence or presence of various concentrations of atorvastatin. After the incubation, the medium was collected and the level of C-reactive protein was measured by enzyme-linked immunosorbent assay. The cells were harvested and C-reactive protein mRNA was analyzed by reverse transcription polymerase chain reaction.
RESULTS:Incubation with lipopolysaccharide significantly induced a time and dose dependent increase in the mRNA expression and the production of C-reactive protein in A549 cells (P<0.05). Atorvastatin significantly decreased the lipopolysaccharide induced the mRNA expression and the production of C-reactive protein in a dose dependent manner in A549 cells (P<0.05).
CONCLUSION:Atorvastatin downregulates lipopolysaccharide-induced expression of C-reactive protein in cultured A549 cells, which may be its mechanism of anti-inflammation.
