Acute cardiac injury activates interleukin-1 beta signaling in the spinal cord.
- Author:
Jianhua LIU
1
;
Di FU
;
Junmei XU
;
Xianghang LU
;
Ruping DAI
Author Information
1. Department of Anesthesia, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Interleukin-1beta;
genetics;
metabolism;
Male;
Myocardial Reperfusion Injury;
chemically induced;
metabolism;
pathology;
Myocardium;
metabolism;
pathology;
RNA, Messenger;
genetics;
metabolism;
Rats;
Rats, Wistar;
Signal Transduction;
Spinal Cord;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2009;34(3):210-215
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of acute cardiac injury on the activation of interleukin-1 beta (IL-1 beta) signaling in the spinal cord.
METHODS:Acute cardiac injury rat model was established by intra-myocardial injection of formalin through diaphragm. IL-1 beta expression was determined by Western blot, immunohistochemistry and in situ hybridization. The DNA binding activities of 2 IL-1 beta transcription factors, activator protein (AP)-1 and nuclear factor kB (NF-kappaB) were measured by electrophoretic mobility shift assay (EMSA).
RESULTS:After cardiac injury, the IL-1 beta protein level was dramatically upregulated in the spinal cord. The upregulated IL-1 beta was mainly expressed in the neurons in the lamina II approximately IV of the spinal cord. In response to cardiac injury, the DNA binding activities of NF-kappaB and AP-1 were greatly activated.
CONCLUSION:Acute cardiac injury could activate the spinal IL-1 beta signaling, which, in turn, may be involved in the progression of heart failure after injury.
