Effect of cordyceps sinensis extract on Klotho expression and apoptosis in renal tubular epithelial cells induced by angiotensin II.
- Author:
Rong TANG
1
;
Qiaoling ZHOU
;
Jinyong SHU
;
Tianfeng TANG
;
Xiang AO
;
Weisheng PENG
;
Yide ZHANG
Author Information
1. Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Angiotensin II;
pharmacology;
Apoptosis;
drug effects;
Caspase 3;
genetics;
metabolism;
Cells, Cultured;
Cordyceps;
chemistry;
Cyclin-Dependent Kinase Inhibitor p21;
genetics;
metabolism;
Drugs, Chinese Herbal;
pharmacology;
Epithelial Cells;
cytology;
metabolism;
Glucuronidase;
genetics;
metabolism;
Humans;
Kidney Tubules;
cytology;
metabolism;
Losartan;
pharmacology;
RNA, Messenger;
genetics;
metabolism;
Tumor Suppressor Protein p53;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2009;34(4):300-307
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of cordyceps sinensis (CS) extract and losartan (Los) on the expression of Klotho (Kl), P53, P21, and apoptosis in renal tubular epithelial cell NRK-52E induced by angiotensin II (Ang II), and to elucidate its therapeutical mechanism in Ang II induced renal tubular epithelial cell apoptosis.
METHODS:NRK-52E cells were incubated with CS with or without Ang II for 24 hours. Experimental groups were divided according to the increasing concentrations of CS:0 (serving as controls), 5, 10, 20, 40, and 80 mg/L. The optimal concentration of CS was selected and cells were divided into 5 groups: controls, Ang II (1*10(-8) mol/L), Ang II (1*10(-8) mol/L)+CS (40 mg/L), Ang II (1*10(-8) mol/L)+Los (1*10(-5) mol/L), and Ang II (1*10(-8) mol/L)+CS (40 mg/L)+Los (1*10(-5) mol/L). After 24 hours, cell proliferation was evaluated by MTT assay. The mRNA and protein expression of Kl, P53 and P21 were measured by RT-PCR. Activity of caspase-3 was evaluated by caspase-3 activity assay Kit. Cell apoptosis was determined by Annexin V-FITC/PI double staining and flow cytometry.
RESULTS:Certain concentrations of CS promoted the proliferation of NRK-52E cells and increased cells proliferation inhibited by Ang II (P<0.01 or P<0.05 ). Ang II significantly down-regulated the mRNA and protein expression of Kl, and up-regulated the levels of P53 and P21. Caspase-3 activity and apoptotic rates were decreased, too (all P values<0.01). CS or/and Los significantly increased the expression of Kl mRNA and protein down-regulated by Ang II, decreased P53 mRNA and protein expression, P21 mRNA and protein expression,and inhibited caspase-3 activity and apoptotic rates(all P values<0.05). No cooperative effects were observed in the two drugs (P>0.05).
CONCLUSION:CS can increase the expression of Kl down-regulated by Ang II, decrease P53 and P21 expression and caspase-3 activity, and reduce Ang II induced NRK-52E cell apoptosis, which may be part of its mechanism of the protective effects on hypertensive renal damage.
