Expression of Foxp3 mRNA in peripheral blood monocytes in patients after kidney transplantation.
- Author:
Cheng ZHOU
1
;
Haitao LIU
;
Hui ZHOU
;
Xiangrong ZHU
;
Xiang DING
;
Zhi CHEN
;
Jinliang XIE
Author Information
1. Department of Transplantation, Xiangya Hospital of Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Biomarkers;
metabolism;
Case-Control Studies;
Female;
Forkhead Transcription Factors;
genetics;
metabolism;
Graft Rejection;
metabolism;
Humans;
Kidney Transplantation;
Male;
Middle Aged;
Monocytes;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Uremia;
blood;
surgery
- From:
Journal of Central South University(Medical Sciences)
2009;34(9):874-878
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between the level of Foxp3 mRNA in the peripheral blood monocytes (PBMCs) with acute rejection or chronic allograft nephropathy after kidney allotransplantation.
METHODS:Real-time quantitative polymerase chain reaction was used to examine Foxp3 mRNA expression in the PBMCs from 16 patients with acute rejection, 8 patients with chronic allograft nephropathy, 8 patients at stable stage after kidney transplantation, 8 patients of end-stage renal disease, and 8 normal controls.
RESULTS:The level of Foxp3 mRNA in the PBMCs was significantly lower in patients with acute rejection than that in patients with chronic allograft nephropathy (P<0.01). Foxp3 mRNA expression was significantly lower in patients with chronic allograft nephropathy than that in the normal controls, the end-stage renal disease group, and stable stage group (P<0.01). The level of Foxp3 mRNA was not significantly different in the PBMCs among the normal controls, the end-stage renal disease group, and the stable stage group (P>0.05).
CONCLUSION:The level of Foxp3 mRNA expressed in PBMCs can reflect the status of renal allograft, and may be a noninvasive biomarker for diagnosing acute rejection and chronic allograft nephropathy.
