Expression of DLK1 gene in acute leukemias and its function in erythroid differentiation of K562 cell line.
- Author:
Xueyuan TANG
1
;
Chan LONG
;
Chenghong WANG
;
Guangfen XIAO
Author Information
1. Department of Hematology, Third Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Adolescent;
Adult;
Aged;
Calcium-Binding Proteins;
Case-Control Studies;
Cell Differentiation;
drug effects;
genetics;
Cell Transformation, Neoplastic;
genetics;
Child;
Child, Preschool;
Erythroid Cells;
pathology;
Erythroid Precursor Cells;
pathology;
Female;
Humans;
Intercellular Signaling Peptides and Proteins;
genetics;
metabolism;
K562 Cells;
Leukemia;
genetics;
metabolism;
Male;
Membrane Proteins;
genetics;
metabolism;
Middle Aged;
RNA, Messenger;
genetics;
metabolism;
Young Adult
- From:
Journal of Central South University(Medical Sciences)
2009;34(9):886-891
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the expression of DLK1 gene in acute leukemias (AL) and its function in erythroid differentiation of K562 cells.
METHODS:We detected the expression of DLK1 gene in 65 different acute leukemia categories (a test group) and 34 normal bone marrow controls (a control group) with RT-PCR. DLK1 protein in 20 out of the 65 AL patients and 13 of the 34 controls was assayed by Western blot. The K562 cell line was induced to erythroid differentiation by hemin. We observed the relationship between its expression and erythroid differentiation.
RESULTS:Both leukemia cells and normal marrow cells expressed DLK1. The expression of DLK1 mRNA in patients in the test group was higher than that in the control group (P=0.018), while there was no significance between acute lymphoblastic leukemia and acute myelogenous leukemia (P>0.05).The expression of DLK1 mRNA in the test group at onset had no relation with the WBC and platelet count in the total peripheral blood, and the same was true for blast cell rates in bone marrow cells.The level of DLK1 protein in the test group was higher than that in the control group, which was consistent with the mRNA expression (P=0.042). The expression of DLK1 mRNA decreased gradually with K562 cells towards hemin-induced erythroid differentiation.
CONCLUSION:DLK1 gene may be involved in leukemia,but the mRNA level of DLK1 has no relation with some clinical characteristics of AL patients at onset. DLK1 may inhibit the erythroid differentiation of K562 cells.
