Preparation and preliminary application of colloidal carbon dipstick for schistosomiasis japonica.
- Author:
Shiping WANG
1
;
Luxin YU
;
Hongli CHE
;
Xiuchun CHEN
;
Yonghua QIN
;
Dongmei GAO
;
Mingshe LIU
Author Information
1. Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China. wsp4373383@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Helminth;
blood;
Carbon;
chemistry;
Colloids;
chemistry;
Humans;
Immunoassay;
methods;
Schistosoma japonicum;
immunology;
isolation & purification;
Schistosomiasis japonica;
blood;
diagnosis;
Sensitivity and Specificity
- From:
Journal of Central South University(Medical Sciences)
2009;34(11):1063-1069
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To develop a rapid and simple immunoassay to detect antibodies in the sera of patients infect Schistosoma japonicum (S. japonicum).
METHODS:Soluble egg antigen (SEA) of S. japonicum conjugated with colloidal carbon in advance was used to react with the antibodies in the sera of patients with schistosomiasis. Then the carbon-antigen-antibody complex would be captured by SEA which had been absorbed on the nitrocellulose membrane and a gray band was shown.
RESULTS:A total of 137 sera samples from S. japonicum epidemic area were tested, and the consistency, sensitivity, and specificity of colloidal carbon dipstick assay were 98.54%, 98.99%, and 97.37%, respectively, compared with the IHA method. The gray scale of bands on the dipstick was curvilinear to serum titer which revealed that the assay could be used semi-quantitatively in serum analysis.
CONCLUSION:Colloidal carbon dipstick assay is not only rapid and simple, but also sensitive and specific for the detection of serum antibodies of schistosomiasis japonica. It will be a practical immunological assay for the diagnosis of schistosomiasis in the field testing.
