Inflammatory cytokine expression in recurrent nasal polyps by antibody chips.
- Author:
Yu CHEN
1
;
Hong SUN
;
Huowang LIU
;
Xuedong CHEN
Author Information
1. Department of Otolaryngology-Head & Neck Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Antibodies;
Chemokine CCL11;
metabolism;
Chemokines;
metabolism;
Cytokines;
metabolism;
Female;
Humans;
Interleukin-1beta;
metabolism;
Interleukin-6;
metabolism;
Male;
Middle Aged;
Nasal Polyps;
metabolism;
Protein Array Analysis;
methods;
Recurrence;
Young Adult
- From:
Journal of Central South University(Medical Sciences)
2009;34(11):1086-1090
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of inflammatory cytokines in patients with recurrent nasal polyps by antibody chips.
METHODS:Proteins from the patients'nasal membrane in a nasal polyps group, a recurrent nasal polyps group, and a control group were labeled with biotin. The biotin-labeled proteins reacted with antibody chips on which the antibodies of 40 major inflammatory cytokines were prepared. The target proteins were conjugated with streptomycin antibody labeled with horseradish peroxidase (HRP),and signals were imaged by laser scanner.
RESULTS:Compared with the control group, the levels of inflammatory cytokines of nasal polyp group were notably increased, including pro-and anti-inflammatory cytokines, chemokines and certain cytokine receptors; while in recurrent nasal polyps, expression of chemokines were increased and most anti-inflammatory cytokines were decreased.
CONCLUSION:Antibody chips demonstrate a significant change in cytokine profiles in patients with recurrent nasal polypsis, as compared with those with nasal polyps. The abnormally higher expression of chemotatic factors in the nasal mucosa may play an important role in the recurrence of human nasal polyps.
