Expression of FGF-2 and osteopontin in non-small cell lung cancer.
- Author:
Ting LI
1
;
Jianhua ZHOU
;
Zhenghao DENG
;
Chunyan FU
;
Haiying JIANG
;
Zhenqin GAO
;
Jinsheng WANG
;
Hongzheng REN
;
Peng WANG
Author Information
1. Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Non-Small-Cell Lung;
metabolism;
pathology;
Female;
Fibroblast Growth Factor 2;
genetics;
metabolism;
Humans;
Lung Neoplasms;
metabolism;
pathology;
Male;
Middle Aged;
Neoplasm Metastasis;
Osteopontin;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2009;34(11):1114-1119
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of fibroblast growth factor-2 (FGF-2) and osteopontin (OPN) in non-small cell lung cancer (NSCLC) tissues and analyze the correlation between FGF-2 and OPN.
METHODS:Immunohistochemical SP method was used to detect the expression of FGF-2 and OPN in 76 patients with NSCLC and 15 normal lung tissues. The effect of FGF-2 on OPN expression at mRNA and protein level in A549 cell was examined by RT-PCR and Western blot.
RESULTS:The positive expression of FGF-2 (65.8%) and OPN (60.5%) in the NSCLC tissues was significantly higher than that in the normal lung tissues (13.3% and 0, respectively ) (P<0.01). The expression of FGF-2 and OPN was closely related to TNM stages and the lymph node metastasis (all Ps<0.01), but not to histological types, sex, and age of NSCLC patients (all Ps>0.05).A positive correlation was found between the expression of FGF-2 and OPN in NSCLC (r=0.552,P<0.01). The expression of OPN protein and mRNA was up-regulated by FGF-2 in A549 cells.
CONCLUSION:The overexpression of FGF-2 and OPN is related to the metastasis and invasion of NSCLC.FGF-2 may promote the metastasis and invasion of NSCLC depending on the upregulation of OPN expression.
