Mouse model of experimental antoimmune encephalomyelitisin C57BL/6J and expression of macrophage migration inhibitory factor.
- Author:
Yun-chun GAO
1
;
Yu-zhong WANG
;
Rui WANG
;
Jin-jin YAN
;
Wen-bin ZHOU
Author Information
1. Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain;
metabolism;
Disease Models, Animal;
Encephalomyelitis, Autoimmune, Experimental;
chemically induced;
metabolism;
Female;
Macrophage Migration-Inhibitory Factors;
biosynthesis;
Mice;
Mice, Inbred C57BL;
Random Allocation;
Up-Regulation
- From:
Journal of Central South University(Medical Sciences)
2008;33(10):931-936
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the expression of macrophage migration inhibitory factor(MIF) in the brain and spinal cord of chronic non-remitting model of experimental autoimmune encephalomyelitis(EAE) mouse, and to discuss their effect on EAE/MS.
METHODS:Seventy-two female SPF C57BL/6J mice, aged 6k8 weeks, were randomly divided into an EAE group, a blank group, and an adjuvant group. The mice were immuned by mMOG35-55 and CFA. Immunohistochemic technique was used to detect the expression of MIF in the brain and spinal cord.
RESULTS:In the EAE group, we observed up-regulation of MIF of central nervous system(CNS) at onset, peak and chronic phase. During each phase, the difference of MIF between the EAE group and each of the other 2 groups was significant (P<0.05). In the EAE group, the expression of MIF was the highest at the peak, which was different from other periods (P<0.05).
CONCLUSION:MIF significanty expressed during the procedure of EAE disease, and may be related with the onset and exacerbation of EAE.
