Molecular Cloning and Nucleotide Sequence of the Gene Encoding Fusion(F) Protein of the Thermostable Newcastle Disease Virus Isolated from a Diseased Pheasant.
- Author:
Kyung Soo CHANG
;
Kui Hyun KIM
;
Moo Hyung JUN
;
Hee Jong SONG
;
Jong Hyeon PARK
- Publication Type:Original Article
- Keywords:
Human rotavirus;
VP7;
RT-PCR;
Nucleotide sequence;
Baculovirus expression
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence*;
Clone Cells;
Cloning, Molecular*;
Cysteine;
DNA, Complementary;
Genotype;
Glycosylation;
Hemagglutination;
Korea;
Newcastle disease virus*;
Newcastle Disease*;
Polymerase Chain Reaction;
RNA;
Texas;
Viral Load
- From:Journal of the Korean Society of Virology
1998;28(3):233-244
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The gene encoding F protein of CBP-1 strain, a heat-stable Newcastle disease virus (NDV) isolated from the diseased pheasants in Korea, was characterized by reverse transcription-polymerase chain reaction (RT-PCR), nucleotide and amino acid sequences. Virus RNA was prepared from the chorioallatoic fluid infected with NDV CBP-1 virus and cDNA was amplified by RT-PCR, cloned and sequenced to analyze. The PCR was sensitive as to detect the virus titer above 25 hemagglutination unit. 1.7kb (1,707bp) size of the cDNA was amplified and cloned into BamHI site of pVL1393 Baculo transfer vector. The nucleotide sequences for F protein were determined by dye terminator cyclic sequencing using four pairs of primers, and 553 amino acid sequences were predicted. In comparison of the nucleotide sequence of F gene of CBP-1 with those of other NDV strains, the homology revealed 88.8%, 98.5% and 98.7% with Kyojungwon (KJW), Texas GB and Beaudette C strains, respectively. As the deduced 553 amino acid sequences of F protein of CBP-1 were compared with those of other NDV strains, the homology appeared 89.9%, 98.7% and 98.9% with KJW, Texas GB and Beaudette C strains, respectively. The putative protease cleavage site (112-116) was R-R-Q-K-R, indicating that CBP-1 strain is velogenic type. The amino acid sequences include 6 sites of N-asparagine-linked glycosylation and 13 cysteine residues. These data indicate that the genotype of CBP-1 strain is more closely associated with the strains of Texas GB and Beaudette C than KJW strain.
