Expression of Class I integrase gene in Acinetobacter baumannii and drug-resistance.
- Author:
Le LI
1
;
Zhong-di XIA
;
Zhao-hui HU
;
Zhi-quan ZHOU
;
Hong-tao LI
Author Information
1. Derpartment of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Acinetobacter Infections;
microbiology;
Acinetobacter baumannii;
genetics;
isolation & purification;
Base Sequence;
Biofilms;
Drug Resistance, Multiple;
genetics;
Humans;
Integrases;
biosynthesis;
genetics;
Molecular Sequence Data;
RNA, Messenger;
biosynthesis;
genetics
- From:
Journal of Central South University(Medical Sciences)
2008;33(10):952-957
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression level of class I integrase (intI 1) mRNA in Acinetobacter baumannii from biofilm cells and planktonic cultured cells ,and to analyze the drug-resistance of Class I integron positive strains.
METHODS:Acinetobacter baumannii were collected from hospitals,and Class I integron strains were screened by gene amplification. Total RNA of Class I integron positive strains was extracted, and the intI1 mRNA expression in the bioflim cells and planktonic cultured cells was measured by RT-PCR. Susceptibilities to antibiotics of Class I integron positive strains were also examined.
RESULTS:The intI1 gene mRNA was expressed under 2 conditions, and the mRNA expressed in the biofilm cells was about 4 times higher than that in the planktonic cultured cells. Among the 64 strains of Acinetobacter baumannii, 46 strains were Class I integron positive strains. The antibiotic resistance of intI1 gene cassette-positive strains was higher than that of gene cassette-negative strains.
CONCLUSION:The intI1 gene mRNA can be up-regulated in Acinetobacter baumannii biofilm cells.Class I integron plays an important role in drug resistance. It is much easier to capture gene cassettes for bacteria under biofilm condition.
