Effect of polygonum multiflorum on the fluidity of the mitochondria membrane and activity of COX in the hippocampus of rats with Abeta 1-40-induced Alzheimer's disease.
- Author:
De-ren HOU
1
;
Yan WANG
;
Li XUE
;
Yi TIAN
;
Kun CHEN
;
Zhi SONG
;
Qi-dong YANG
Author Information
1. Department of Neurology, Third Xiangya Hospital, Central South University, Changsha 410013, China. hou0718@xy3yy.com
- Publication Type:Journal Article
- MeSH:
Alzheimer Disease;
chemically induced;
metabolism;
Amyloid beta-Peptides;
Animals;
Cyclooxygenase 1;
metabolism;
Disease Models, Animal;
Drugs, Chinese Herbal;
pharmacology;
Female;
Hippocampus;
metabolism;
pathology;
Male;
Membrane Fluidity;
drug effects;
Membrane Proteins;
metabolism;
Mitochondria;
drug effects;
Peptide Fragments;
Polygonum;
chemistry;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2008;33(11):987-992
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore the effect of polygonum multiflorum on the fluidity of mitochondria membrane and activity of cytochrome oxidase (COX) in Alzheimer's disease (AD) model rats.
METHODS:Forty-five SD rats were randomly divided into 3 groups: an AD model group, a control group, and a treatment group (n=15). AD model was established by injecting beta-amyloid protein (Abeta) 1-40 into the hippocampus of rats. The learning and memory abilities of rats were tested with the Y-electrical maze. The coefficient of viscosity of the hippocampal mitochondria membrane was determined by a spectrofluorometer, and the activity of COX was measured by an ultraviolet spectrophotometer.
RESULTS:Compared with the control group, the learning and memory ability of the AD model group was significantly lower (P<0.01), while the coefficient of viscosity of the hippocampal mitochondria membrane of the AD model group rats was significantly higher (P<0.01), and COX activity was lower (P<0.01). Compared with the AD model group rats, the coefficient of viscosity of the hippocampal mitochondria membrane of the treatment group was significantly lower (P<0.05), and COX activity was significantly improved (P<0.05).
CONCLUSION:Polygonum multiflorum could improve the fluidity of mitochondria membrane and the activity of mitochondrial COX in the model of Alzheimer's disease.
