Activation of anti-tumor cytotoxic T lymphocytes by fusion of human dendritic cells and melanoma cells.
- Author:
He-cheng ZHU
1
;
Shang-hui WU
;
Bai-ying HUANG
;
Bin ZHU
;
Huan-hua GU
;
Xue-bin LUO
;
Zhuang XIONG
;
Wei-san CHEN
Author Information
1. Department of Cell Biology, Central South University, Changsha 410078, China. zhuhecheng1954@yahoo.com
- Publication Type:Journal Article
- MeSH:
Antigen Presentation;
immunology;
Antigens, Neoplasm;
immunology;
Cancer Vaccines;
immunology;
Cell Fusion;
Cell Line, Tumor;
Dendritic Cells;
cytology;
immunology;
HLA-A2 Antigen;
immunology;
Humans;
MART-1 Antigen;
immunology;
Melanoma;
immunology;
pathology;
T-Lymphocytes, Cytotoxic;
immunology
- From:
Journal of Central South University(Medical Sciences)
2008;33(11):1012-1018
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of activation of specific anti-tumor cytotoxic T lymphocytes (CTL) and the ability of cross-presentation in vitro by fusion of HLA-A2+ human dendritic cells (DCs) with HLA-A2- melanoma cells.
METHODS:The HLA-A2+ human dendritic cells and HLA-A2- melanoma cells were fused by PEG and were cultivated in complete RPMI1640 media containing FCS (10%) and GM-CSF for 24-48 h, and then co-cultured fusion cells with Melan-A specific T cells. HLA-A2- melanoma cells were negative control,While T2 cells and DC+Pts were positive control. The activation of anti-tumor CTL elicited by the fusion cells was detected by intracellular cytokine staining.
RESULTS:The immature DC could express CD80, CD83, CD86, HLA-DR, and HLA-ABC,but the mature DC induced by TNF-alpha, PGE-2, and CD40L further highly expressed above molecules. The rate of specific CTL cells primed by the fusion cells was 16.72%+/-4.26%, negative control was 0.21%+/-1.84%,and positive control was 28.60%+/-5.67%. The CTL from vaccine by fusing DC and LAR6 induced lysis of HLA-A2+ LAR1 cells.
CONCLUSION:The HLA-A2 restricted specific anti-tumor CTL can be induced in vitro by fusion of HLA-A2+ human dendritic cells with HLA-A2- melanoma cells.
