Expression of EBI3 and p28 mRNA in the brain and spinal cord of chronic model of experimental autoimmune encephalomyelitis in C57BL/6J mice.
- Author:
Jin-jin YAN
1
;
Rui WANG
;
Yu-zhong WANG
;
Wen-bin ZHOU
Author Information
1. Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain;
metabolism;
Chronic Disease;
Encephalomyelitis, Autoimmune, Experimental;
metabolism;
Interleukins;
genetics;
metabolism;
Male;
Mice;
Mice, Inbred C57BL;
Minor Histocompatibility Antigens;
Protein Subunits;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Receptors, Cytokine;
genetics;
metabolism;
Spinal Cord;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2008;33(11):1028-1036
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of EBI3 and p28 mRNA (the 2 subunits of IL-27) in the brain and spinal cord of the model of experimental autoimmune encephalomyelitis (EAE), and to explore their effect on EAE.
METHODS:Seventy-two adult female SPF C57BL/6J mice (inbred strain) were randomly divided into a control group, an adjuvant group, and an EAE group. RT-PCR was performed to detect the expression of EBI3 mRNA and p28 mRNA in the brain and spinal cord.
RESULTS:The expression of EBI3 mRNA and p28 mRNA was up-regulated at onset in the EAE group, which increased quickly during peak phase and maintained at a high level in the chronic phase. There was significant difference in the expression of EBI3 and p28 mRNA between the EAE group and the control/adjuvant group (P<0.01). Additionally, there was no remarkable difference in the expression of EBI3 and p28 mRNA in the brain and spinal cord between the control group and the adjuvant group (P>0.05).
CONCLUSION:IL-27 may play a role of promoting the morbility of EAE in the early stage, and sustain the inflammatory response in endgame.
