Mapping of Human Cytomegalovirus IE1 Responsive Elements in the c-jun Promoter.
- Author:
Dae Joong KIM
;
Jin Hee KIM
;
Sung Bae CHOI
;
Tae Hee HAN
;
Chung Gyu PARK
;
Eung Soo HWANG
;
Chang Yong CHA
- Publication Type:Original Article
- Keywords:
HCMV;
Immediate early proteinl;
c-jun promoter;
CTF
- MeSH:
Binding Sites;
Cytomegalovirus*;
Fibroblasts;
Humans*;
Immediate-Early Proteins;
Luciferases;
Lung;
Plasmids;
Promoter Regions, Genetic;
Trans-Activators;
Transcription Factor AP-1;
Transcription Factors;
Up-Regulation;
Viral Proteins
- From:Journal of the Korean Society of Virology
1998;28(3):267-274
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Human cytomegalovirus (HCMV) has the ability to activate the espremission of many viral and cellular genes. Among various viral proteins, the immediate early proteins (IE1-72kDa, IE2-86kDa) have been known to be potent transactivators. The product of c-jun photo-oncogene is important in cell activation and differentiation. Here, we tried to find out if the IE could activate the c-jun promoter and also tried to identify the responsible sequence elements in the c-jun activation by IE1-72kDa. We found HCMV IE expression transactivated the c-jun promoter in human embryonal lung fibroblasts (HEL). The activation fold by IE1-72kDa, IE2-86kDa and IE2-55kBa was 23, 35, and 5, respectively. When the expression of each IE was combined, it showed synergism. Expression of (IE1-72kDa + IE2-86kBa) and (IE1-72kDa + IE2-86kDa + IE2-55kDa) resulted in 131 and 162 fold increase, respectively. The c-jun promoter region between -117 and -59 contains binding sites for the transcription factors Spl, CAAT, AP-1 like (ATF/CREB), and MEF2. Transient expression assays were performed using various reporter plasmids containing the c-jun promoter-regulatory region linked to the luciferase gene and a plasmic expressing HCMV IE1 gene. Deletional and point mutational analysis showed that the sequence between -225 to -160 and the CTF binding site were involved in the up-regulation of c-jun promoter.
