Effect of suppressive oligodeoxynucleotides on the functional differentiation in CD4(+) Th1 lymphocytes in mice in vitro.
- Author:
Sha-Ling LI
1
;
Xia-Hong DAI
;
Xue-Gong FAN
Author Information
1. Department of Infection , Xiangya Hospital, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Animals;
CD4-CD8 Ratio;
CD4-Positive T-Lymphocytes;
cytology;
immunology;
Cell Differentiation;
drug effects;
Female;
Interferon-gamma;
antagonists & inhibitors;
physiology;
Interleukin-12;
antagonists & inhibitors;
physiology;
Mice;
Mice, Inbred BALB C;
Oligodeoxyribonucleotides;
pharmacology;
T-Lymphocyte Subsets;
Th1 Cells;
cytology;
immunology
- From:
Journal of Central South University(Medical Sciences)
2008;33(12):1089-1094
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of suppressive oligodeoxynucleotides (Sup ODN) on the Th1 differentiation of CD4(+)T splenetic lymphocytes in mice.
METHODS:The splenetic lymphocytes of BALB/c mice were separated, and then CD4(+) cells were purified with immune magnetic CD4(+) microbeads (positive selection). The purification was examined by fluorescence-activated cell sorter. CD4(+) cells, anti-CD3epsilon, anti-CD28, IL-12 and Sup ODN or control oligodeoxynucleotides (Con ODN) were co-incubated for 72 h. IFN-gamma and IL-4 in the supernatant were detected using enzyme-linked immunosorbent assay. The expression of T-bet mRNA in CD4(+) cells was tested by reverse transcription polymerase chain reaction.
RESULTS:Sup ODN could significantly inhibit the release of INF-gamma and increase IL-4 production respectively (P<0.01). T-bet mRNA of CD4(+) lymphocytes was remarkably inhibited by Sup ODN as well (P<0.01).
CONCLUSION:In the presence of pro-Th1-cytokines, Sup ODN may affect the differentiation of CD4(+) T lymphocytes in vitro. Sup ODN can promote CD4(+) T cells to differentiate into Th2, and suppress them into Th1.
