Construction of WISP3 gene's mutants in SEDT-PA and their expression in COS-7 cells.
- Author:
Min WANG
1
;
Yi-qun PENG
;
Hou-de ZHOU
;
Mu-xu ZHAI
;
Yu-ling HE
;
Hui XIE
Author Information
1. Institute of Metablism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011,China.
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
CCN Intercellular Signaling Proteins;
COS Cells;
metabolism;
Chlorocebus aethiops;
Humans;
Insulin-Like Growth Factor Binding Proteins;
biosynthesis;
genetics;
Molecular Sequence Data;
Mutagenesis, Site-Directed;
Mutation;
Osteochondrodysplasias;
genetics;
metabolism;
Transfection
- From:
Journal of Central South University(Medical Sciences)
2008;33(1):8-15
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct two types of Wnt-inducible secreted protein 3(WISP3) gene's mutants(1000T/C,840delT) found in spondyloepiphyseal dysplasia tarda with progressive anthopathy (SEDT-PA) patients, and to observe their expression in COS-7 cells.
METHODS:Full-length cDNA of wild type WISP3 gene(WT-WISP3) was amplified from human chondrocytes by RT-PCR, and site-directed mutagenesis was used to obtain full-length cDNAs of the mutated WISP3 genes(MUT1000T/C and MUT840delT). The recombined plasmids WT-WISP3/pcDNA3.1(+), MUT1000T/C/pcDNA3.1(+) and MUT840delT/pcDNA3.1(+) were transfected transiently into COS-7 cells by liposome-mediated method, and pcDNA3.1(+) vector was used as a control. The total RNA and protein of the transfected COS-7 cells were extracted after 48 hours of transfection. The expression of WISP3 gene in the transfected COS-7 cells was detected by semi-quantitative RT-PCR and Western blot.
RESULTS:By restriction endonuclease analysis and sequencing, the sequence of MUT1000T/C and MUT840delT were consistent with that mutated in SEDT-PA, and the open reading frames matched with the vector sequence. Semi-quantitative RT-PCR and Western blot showed that the recombined plasmids were highly expressed in COS-7 cells.
CONCLUSION:WISP3 gene's mutants of SEDT-PA are successfully constructed by genetic recombination, and expressed in COS-7 cells, which lays the foundation for the further study on its molecular functions in SEDT-PA.
