Killing effect improved by fusion gene HRE1.Egr-1. yCDglyTK on gene-radio therapy of nasopharyngeal cancer in vitro.
- Author:
Yu ZHONG
1
;
Yao-yun TANG
;
Chang-ning XIE
;
Su-ping ZHAO
Author Information
1. Department of Otolaryngology, Xiangya Hospital,Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Early Growth Response Protein 1;
genetics;
Flucytosine;
pharmacology;
Gene Fusion;
physiology;
Genetic Therapy;
methods;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
genetics;
Nasopharyngeal Neoplasms;
genetics;
radiotherapy;
therapy;
Response Elements;
genetics;
Thymidine Kinase;
genetics;
metabolism;
Tumor Cells, Cultured
- From:
Journal of Central South University(Medical Sciences)
2008;33(2):110-114
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct hypoxia/radiation inducible promotor HRE1.Egr-1, and to observe its promotive effect on the expression of yCDglyTK gene in nasopharyngeal cancer HNE-1 cells and the anti-tumor effect of yCDglyTK and to lay an experimental foundation for further exploration of new gene-radio therapy of nasopharyngeal cancer.
METHODS:pcDNA3.1(-)HRE1.Egr-1.yCDglyTK was constructed by gene recombination technique. Stable yCDglyTK-expressing HNE-1 cells were generated by transfecting the recombinant plasmid into the target cells with liposome. The expression of yCDglyTK was detected by Western blot in 4 groups: a normoxia group, a radiation group, a hypoxia group, and a hypoxia and radiation group. The killing effect of 5-FC in different circumstances was determined by MTT.
RESULTS:The expression of yCDglyTK/5-FC gene in all the groups was significantly different(P<0.01),especially in the hypoxia and radiation group. The killing effect of 5-FC on HNE1 cells varied under different conditions, especially in the hypoxia and radiation group.
CONCLUSION:Hypoxia and radiation can induce the activity of fusion promoter HRE1.Egr-1, and obviously promote the anti-tumor effect of yCDglyTK/5-FC system, suggesting that yCDglyTK may be a candidate suicide gene for gene-radio therapy of NPC.
