Effect of IL-10 on LOX-1 expression of peripheral blood monocytes in patients with acute coronary syndrome.
- Author:
Jian-gang WANG
1
;
Zhong-shu LIANG
;
Kan YANG
;
Zhi-jun HUANG
;
Jing-le LI
Author Information
1. Department of Cardiology, Third Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Acute Coronary Syndrome;
blood;
Aged;
Cells, Cultured;
Female;
Humans;
Interleukin-10;
metabolism;
pharmacology;
Male;
Middle Aged;
Monocytes;
cytology;
metabolism;
RNA, Messenger;
biosynthesis;
genetics;
Scavenger Receptors, Class E;
biosynthesis;
genetics
- From:
Journal of Central South University(Medical Sciences)
2008;33(2):169-173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) of peripheral blood monocytes in patients with acute coronary syndromes (ACS),and to further explore the effect of anti-inflammatory factor interleukin-10 (IL-10) on the expression of LOX-1.
METHODS:Twenty-eight healthy controls and 28 ACS patients were enrolled in the study. The levels of IL-10 and tumor necrosis factor (TNF-alpha) were determined by enzyme-linked immunosorbnent assay(ELISA). The monocytes of peripheral blood in patients and controls were isolated and incubated with exogenous IL-10 (20 microg/L). The expression of LOX-1 protein and mRNA in the monocytes was examined by Western blot and reverse transcriptase PCR (RT-PCR).
RESULTS:Compared with the healthy controls, the levels of serum IL-10 and TNF-alpha were significantly elevated in ACS patients (P<0.01). The expression of LOX-1 protein and mRNA was markedly upregulated in the isolated monocytes from ACS patients, which could be downregulated by IL-10 (20 microg/L, 3 h) (P<0.01).
CONCLUSION:The effect of anti-inflammatory factor IL-10 on the atherosclerosis may be a new mechanism resulting in plaque stabilization via the decreased LOX-1 expression of peripheral monocytes in ACS patients.
